Checked my first crystallization plates today. Lots of precipitant; 70% methanol was not the answer (only 1/4 of wells down, no biggie). No crystals in most of the other wells yet, except one. One that I messed up.
That particular well I didn't seal very well, so it dehydrated. This concentrates the protein, inducing crystallization as the solution grow saturated. These might be usable for microseeding future trials.
The other wells may still form crystals, given some time. The other answer may be to poke holes in the seals and let them dehydrate too. The real answer is do it all over again and use a higher concentration.
Being science, will try all of them, as only one really needs to work.
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